Protran® membranes are manufactured from 100% pure nitrocellulose to ensure the highest binding capacity possible
- High binding, low background, without the need for stringent washing conditions
- Proteins maintain molecular recognition activity for many years
The 0,2 µm pore size membrane (Protran® BA83) ensures high retention of small proteins below 20 kD by reducing ‘blow-through’ and the 0,45 µm pore size membrane (Protran® BA85) is ideal for larger molecular weights.
Protran® binding capacity: 80 – 150 µg/cm²
They are compatible with a variety of detection methods, including isotopic, chemiluminescent, colorimetric and fluorescent. No methanol pre-wetting step is required, making this the membrane of choice for proteins which prefer aqueous environments. Prior to transfer the membrane is simply wetted with water, and then placed in the transfer buffer. No other pre treatment steps are necessary.
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